Western Blotting - Bio-Rad
Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights starting from a mixture of proteins extracted from cells or tissues. Stain-Free Western Blotting Enables Faster …
Bio-Rad Laboratories, Inc. Life Science Group General Protocol for Western Blotting Protein separation by gel electrophoresis 1. Load equal amounts of protein (20 μg) into the wells of a mini (8.6 x 6.7 cm) or midi (13.3 x 8.7 cm) format SDS-PAGE gel, along with molecular weight markers. 2. Run the gel for 5 min at 50 V. 3.
Introduction to Western Blotting - Bio-Rad
Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights. All from a starting mixture of proteins extracted from cells or tissues.
Western Blotting Workflow Products - Bio-Rad
Explore Bio-Rad's complete solutions for your western blotting workflow - gels, electrophoresis chambers, western blotting transfer and imaging systems, buffers, membranes, and immunodetection reagents and kits.
Western Blot Protocol - Bio-Rad
This video demonstrates SDS-PAGE separation of proteins using the Bio-Rad Comparative Proteomics Kit II: Western Blot Module. Assembly of the blotting sandwich and electroblotting are shown along with the steps for protein detection using a colorimetric assay.
Introduction to Western Blotting - Bio-Rad
Western blotting, also known as immunoblotting is a core technique in cell and molecular biology. Our guide provides an overview for performing successful Western blots.
Fundamentals of Western Blotting Sample Preparation - Bio-Rad …
Learn how western blots are used in biological research, experimental design considerations before you begin your project, and reagents and methods for good sample preparation.
Advanced Western Blotting Sample Preparation - Bio-Rad …
These advanced western blotting courses provide more comprehensive information on the topics that were first discussed in the fundamentals courses. In this course, you will learn about these sample preparation topics: protein sources, cell lysis, sample preparation buffers, and reduction.
All Courses - Bio-Rad Academy
Take a deep dive into advanced western blotting sample preparation. In this course you will learn about: protein sources, cell lysis, sample preparation buffers, and reduction.
Quick Tips for Western Blotting - Bioradiations
Feb 14, 2023 · Learn how to improve your western blotting results with Bio-Rad’s Quick Tips video series. These 1–2 minute videos show you how to perfect each step of the western blotting process and were designed to accompany the in-depth curriculum available in the Western Blot Learning Center.