Consensus and variations in cell line specificity among human ...
Apr 23, 2019 · Human metapneumovirus (HMPV) has been a notable etiological agent of acute respiratory infection in humans, but it was not discovered until 2001, because HMPV replicates …
Consensus and variations in cell line specificity among human ...
Apr 23, 2019 · Recently, the human malignant melanoma MNT-1 cell line has been demonstrated to have a clear CPE upon infection with HMPV . To promote the study of HMPV, several …
Efficient isolation of human metapneumovirus using MNT‐1, a …
Sep 20, 2017 · We isolated HMPV from 102 samples (2.5%) through CPE detection, but only in LLC‐MK2 and MNT‐1 cells. In contrast, we did not isolate enteroviruses, rhinoviruses or …
Studies of culture conditions and environmental stability of human ...
One brief report described limited data on HMPV persistence on environmental surfaces, but the data were based on qualitative RT-PCR and observation for HMPV CPE in culture for five …
Outbreak of Human Metapneumovirus Detected by Use of the …
We found the CPE for hMPV in a primary culture within 6 to 7 days after specimen inoculation for 6 strains, within 8 to 14 days for 46 strains, within 15 to 21 days for 23 strains, and after more …
Efficient isolation of human metapneumovirus using MNT-1, a ... - PubMed
Isolation of human metapneumovirus (HMPV) from clinical specimens is currently inefficient because of the lack of a cell culture system in which a distinct cytopathic effect (CPE) occurs. …
Consensus and variations in cell line specificity among human ...
Apr 23, 2019 · In this study, we re-evaluated and compared the cell line specificity of clinical isolates of HMPV strains, including the novel HMPV A2b180nt-dup strains, and six …
Human metapneumovirus (HMPV) has been detected in patients with acute respiratory infection in North America, South America, Europe, Asia, the Middle East, Africa, and Oceania (1–7).
Analysis of Replication Kinetics of the Human Metapneumovirus …
Jan 1, 2005 · Using a quantitative real-time TaqMan PCR, we analyzed the replication kinetics of hMPV in different cell lines. Our results indicate that hMPV replicates slightly more efficiently in …
Ten years of human metapneumovirus research - ScienceDirect
Feb 1, 2012 · The hMPV-induced CPE is characterized by a rounding of infected cells, subsequent detachment from cell culture matrix and occasionally the presence of small …