Label-free, gel-free, and antibody-free quantitation of Ras from complex mixture targeting exclusively proteoform-specific tryptic peptides detected in 3D-cultured and 2D-cultured NCI-H23 cells.
The vast majority of peptide-centric applications incorporate trypsin and search for tryptic peptides, but other enzymes or cleavage agents can be employed. However, searching for nontryptic ...
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